The doctoral task: To study the effect of ETS exposure on plasma and nasal epithelial cell proteome of workers from those establishments by proteomic-based techniques. The identification of differentially expressed proteins on these ETS exposed cells/tissue could provide new candidate biomarkers to better predict/assess respiratory diseases associated to ETS exposure. The candidate will develop and apply protein/peptide separation and mass spectrometry (MS) techniques, namely two-dimensional gel electrophoresis (2DE), MALDI-TOF-TOF and LC/MS/MS. Bioinformatic tools and molecular biology techniques will be used to investigate and validate putative protein biomarkers. The candidate should be available for short lab-training abroad (2-6 months).
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